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hplc ultimate 3000  (Thermo Fisher)


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    Structured Review

    Thermo Fisher hplc ultimate 3000
    Hplc Ultimate 3000, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hplc+ultimate+3000/q+exactive+mass+spectrometer/10__22175_slash_mmb__17305-74-41-39
    Average 90 stars, based on 1 article reviews
    hplc ultimate 3000 - by Bioz Stars, 2026-09
    90/100 stars

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    Article Title: Tryptophan-producing bacteria to mitigate osteoporosis and intestinal dysfunction
    Article Snippet: High-performance liquid chromatography (HPLC; Thermo, UltiMate 3000) was used to determine tryptophan and butyrate in the bacterial supernatants.

    Article Title: Targeted nanoparticle for the treatment of traumatic brain injury and other CNS diseases
    Article Snippet: Optimization and Validation of HPLC Settings and Acquisition Conditions Prior to the encapsulation efficiency and drug release study, the HPLC method was optimized and validated on a Thermo Fisher UltiMate 3000 HPLC, equipped with a photodiode array detector.

    Article Title: Particulate Matter and Associated PAHs within the Fumes Emitted from Heat-Cooking Rapeseed Oils: Focus on the Refining Level and Trace Components of Rapeseed Oil.
    Article Snippet: The refining process can improve the edible oil’s smoke point and reduce the heat-cooking fume emissions.. However, the influence of temperature, refining level, and trace components within rapeseed oil on particulate matter (PM) and its associated polycyclic aromatic hydrocarbons (PPAHs) in the heat-cooking fumes remains unclear.. Herein, the effects of the refining degree and trace components of rapeseed oil on PM and PPAHs in heat-cooking fumes were analyzed.

    Article Title: Title Pending 17305
    Article Snippet: The objective of this study was to identify proteins and biochemical pathways associated with beef color and tenderness from Angus steers fed graded levels of sorghum-based finisher diets.. Nine 8-month-old (230 ± 28 kg average initial weight) Angus steers were individually housed in pens and randomly allocated to one of the 3 dietary treatments (n= 3) for 90 d of feeding.. The pellet diets were formulated by replacing white maize in the basal diet with either 0 (control, SGD-0), 200 (SGD-200), or 400 (SGD-400) g/kg dry matter of sorghum grain.

    Article Title: Extruded chokeberry pomace as valuable by-product: Effect of extrusion-cooking conditions on composition and nutritional potential
    Article Snippet: The fat-soluble vitamins (FSV) (tocopherols, E; phylloquinone, K1; ergocalciferol, D2; menaquinone, K2) and water-soluble vitamins (WSV) (thiamin, B1; riboflavin, B2; niacin and niacinamide, B3; pantothenic acid, B5; pyridoxin, B6; folate, B9) contents of chokeberry pomace and the extrudates were determined after extraction performed according to the method of Santos, Mendiola, Oliveira, Ibáñez, and Herrero (2012), using high performance liquid chromatography technique coupled with tandem mass spectrometry and electrospray ionization (HPLC-ESI-MS/MS; HPLC Ultimate 3000 ThermoFisher Scientific with LC-MS/MS 4500 QTRAP mass spectrometer, ABSciex, Framingham, MA, USA) as described by Lee et al. (2017).

    Article Title: In-situ synthesis of hydrotalcite-rich porous geopolymers from landfill leachate concentrate for CO2 capture
    Article Snippet: Carbamazepine was detected by high-performance liquid chromatography (HPLC, Ultimate 3000, Thermo Fisher, USA) using a C18 reverse phase chromatography column (4.6 × 250 mm, 5 μm, Waters) (Zhang, Y. et al., 2021).

    High Performance Liquid Chromatography:

    Article Title: Efficiency of surfactant extraction of pigments from wet and non-disrupted Chlorella sorokiniana produced in a bioenergy façade
    Article Snippet: The efficiency of the extraction of pigments from Chlorella sorokiniana produced in a bioenergy façade (53 29′ 42’’ N 10 0′ 40’’ O) with the surfactant Heptylglycerin was studied.. Samples were obtained in monthly intervals between May and November 2022 and microalgae were extracted with surfactant: biomass ratios between 3.2 and 5.9, extraction times down to 1 h and temperatures <60 C. The pigment content of the microalgae was mainly controlled by PAR and varied by a factor of up to 10 during the study period whereby Carotin, Violaxanthin and Zeaxanthin contents decreased with increase in PAR prevailing during a period of 7 days.. Yields of extraction of pigments with Heptylglycerin correlated linearly with their contents in biomass.

    Article Title: Improvement of the rheological properties and performance of liquid crystal emulsions: influence of oil addition and/or high-frequency ultrasound treatment.
    Article Snippet: .. 2.3.6 Quantification of Vitamin C by High-Performance Liquid Chromatography (HPLC) HPLC analysis was performed on an Ultimate 3000 (Thermo Fisher Scientific, Les Ulis, France) using Chromeleon 7.1 software. .. The system was equipped with a DAD UV Ultimate 3000 detector and a SUPELCOSILTM LC-NH2 column with a particle size of 5 μm and a length of 4.6 × 150 mm (Sigma Aldrich, Saint-Quentin-Fallavier, France).

    Fluorescence:

    Article Title: Efficiency of surfactant extraction of pigments from wet and non-disrupted Chlorella sorokiniana produced in a bioenergy façade
    Article Snippet: The efficiency of the extraction of pigments from Chlorella sorokiniana produced in a bioenergy façade (53 29′ 42’’ N 10 0′ 40’’ O) with the surfactant Heptylglycerin was studied.. Samples were obtained in monthly intervals between May and November 2022 and microalgae were extracted with surfactant: biomass ratios between 3.2 and 5.9, extraction times down to 1 h and temperatures <60 C. The pigment content of the microalgae was mainly controlled by PAR and varied by a factor of up to 10 during the study period whereby Carotin, Violaxanthin and Zeaxanthin contents decreased with increase in PAR prevailing during a period of 7 days.. Yields of extraction of pigments with Heptylglycerin correlated linearly with their contents in biomass.

    Software:

    Article Title: Improvement of the rheological properties and performance of liquid crystal emulsions: influence of oil addition and/or high-frequency ultrasound treatment.
    Article Snippet: .. 2.3.6 Quantification of Vitamin C by High-Performance Liquid Chromatography (HPLC) HPLC analysis was performed on an Ultimate 3000 (Thermo Fisher Scientific, Les Ulis, France) using Chromeleon 7.1 software. .. The system was equipped with a DAD UV Ultimate 3000 detector and a SUPELCOSILTM LC-NH2 column with a particle size of 5 μm and a length of 4.6 × 150 mm (Sigma Aldrich, Saint-Quentin-Fallavier, France).



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    Image Search Results


    Identification of the active compound in Saponaria officinalis . ( a ) Western blot analysis of the ability of HPLC fractions of the ethanolic extract of Saponaria officinalis to induce autophagy (LC3 I/II staining: the panel shows the fragment containing the active fraction; the full image is presented in ; densitometric analysis of the LC3 II/LC3 I ratio is shown under the staining result; the fraction sent for further analysis is marked in bold). ( b ) MS/MS comparison of the observed ion in positive-ion mode (top graph, m / z 595.156) with the deposited GNPS reference spectrum for saponarin [M + H] + (bottom graph, GNPS LIBRARY: accession: CCMSLIB00010125140, m / z 595.1660). ( c ) Chemical structure of saponarin.

    Journal: Metabolites

    Article Title: HR-LCMS/MS-Based Dereplication of Plant-Derived Autophagy Inducers Revealed Astragalus dasyanthus as a New Glabrol Producer

    doi: 10.3390/metabo16050311

    Figure Lengend Snippet: Identification of the active compound in Saponaria officinalis . ( a ) Western blot analysis of the ability of HPLC fractions of the ethanolic extract of Saponaria officinalis to induce autophagy (LC3 I/II staining: the panel shows the fragment containing the active fraction; the full image is presented in ; densitometric analysis of the LC3 II/LC3 I ratio is shown under the staining result; the fraction sent for further analysis is marked in bold). ( b ) MS/MS comparison of the observed ion in positive-ion mode (top graph, m / z 595.156) with the deposited GNPS reference spectrum for saponarin [M + H] + (bottom graph, GNPS LIBRARY: accession: CCMSLIB00010125140, m / z 595.1660). ( c ) Chemical structure of saponarin.

    Article Snippet: The analysis was performed using an UltiMate 3000 Acclaim RSLC HPLC system (Fisher Scientific, Waltham, MA, USA) with a 120 C18 2.2 um 2.1 × 100 mm column and a gradient of acetonitrile (0.1% FA) in water (0.1% TFA) of 5–95% for 10 min, while connected to a Bruker maXis II 4 G ETD (Bruker, Billerica, MA, USA).

    Techniques: Western Blot, Staining, Tandem Mass Spectroscopy, Comparison

    Identification of the active compounds in Veronica officinalis . ( a ) Western blot analysis of the ability of HPLC fractions of the ethanolic extract of Veronica officinalis to induce autophagy (LC3 I/II staining: the panel shows the fragment containing the active fraction; the full image is presented in , and densitometric analysis of the LC3 II/LC3 I ratio is shown under the staining result; the fraction sent for further analysis is marked in bold). ( b ) MS/MS comparison of the observed ions in positive-ion mode ( m / z 447.091) and negative-ion mode ( m / z 445.076) with the deposited GNPS reference spectra for baicalin [M + H] + and [M − H] − (GNPS-LIBRARY: accession: CCMSLIB00013029693, m / z 447.092; GNPS-LIBRARY: accession: CCMSLIB00012855511, m / z 445.078, respectively). ( c ) Chemical structure of baicalin.

    Journal: Metabolites

    Article Title: HR-LCMS/MS-Based Dereplication of Plant-Derived Autophagy Inducers Revealed Astragalus dasyanthus as a New Glabrol Producer

    doi: 10.3390/metabo16050311

    Figure Lengend Snippet: Identification of the active compounds in Veronica officinalis . ( a ) Western blot analysis of the ability of HPLC fractions of the ethanolic extract of Veronica officinalis to induce autophagy (LC3 I/II staining: the panel shows the fragment containing the active fraction; the full image is presented in , and densitometric analysis of the LC3 II/LC3 I ratio is shown under the staining result; the fraction sent for further analysis is marked in bold). ( b ) MS/MS comparison of the observed ions in positive-ion mode ( m / z 447.091) and negative-ion mode ( m / z 445.076) with the deposited GNPS reference spectra for baicalin [M + H] + and [M − H] − (GNPS-LIBRARY: accession: CCMSLIB00013029693, m / z 447.092; GNPS-LIBRARY: accession: CCMSLIB00012855511, m / z 445.078, respectively). ( c ) Chemical structure of baicalin.

    Article Snippet: The analysis was performed using an UltiMate 3000 Acclaim RSLC HPLC system (Fisher Scientific, Waltham, MA, USA) with a 120 C18 2.2 um 2.1 × 100 mm column and a gradient of acetonitrile (0.1% FA) in water (0.1% TFA) of 5–95% for 10 min, while connected to a Bruker maXis II 4 G ETD (Bruker, Billerica, MA, USA).

    Techniques: Western Blot, Staining, Tandem Mass Spectroscopy, Comparison

    Identification of the active compounds in Astragalus dasyanthus . ( a ) Western blot analysis of the ability of HPLC fractions of the ethanolic extract of Astragalus dasyanthus to induce autophagy (LC3 I/II staining: the panel shows the fragment containing the active fraction; the full image is presented in ; the fraction sent for further analysis is marked in bold). ( b ) MS/MS comparison of the observed ion in negative-ion mode ( m / z 391.1910) with the reference spectrum for glabrol [M − H] − (GNPS-LIBRARY; accession: CCMSLIB00005749603, m / z 393.19). ( c ) Chemical structure of glabrol.

    Journal: Metabolites

    Article Title: HR-LCMS/MS-Based Dereplication of Plant-Derived Autophagy Inducers Revealed Astragalus dasyanthus as a New Glabrol Producer

    doi: 10.3390/metabo16050311

    Figure Lengend Snippet: Identification of the active compounds in Astragalus dasyanthus . ( a ) Western blot analysis of the ability of HPLC fractions of the ethanolic extract of Astragalus dasyanthus to induce autophagy (LC3 I/II staining: the panel shows the fragment containing the active fraction; the full image is presented in ; the fraction sent for further analysis is marked in bold). ( b ) MS/MS comparison of the observed ion in negative-ion mode ( m / z 391.1910) with the reference spectrum for glabrol [M − H] − (GNPS-LIBRARY; accession: CCMSLIB00005749603, m / z 393.19). ( c ) Chemical structure of glabrol.

    Article Snippet: The analysis was performed using an UltiMate 3000 Acclaim RSLC HPLC system (Fisher Scientific, Waltham, MA, USA) with a 120 C18 2.2 um 2.1 × 100 mm column and a gradient of acetonitrile (0.1% FA) in water (0.1% TFA) of 5–95% for 10 min, while connected to a Bruker maXis II 4 G ETD (Bruker, Billerica, MA, USA).

    Techniques: Western Blot, Staining, Tandem Mass Spectroscopy, Comparison

    Screening of glucuronosyltransferases in betanin-producing S. cerevisiae . a HPLC-UV chromatogram, comparing the total fraction of the parent strain ST12160 with the strains expressing Ah AmaSy1, Cq AmaSy1 or Cc AmaSy1 and the plant extract from B. glabra . A compound with an RT of 5.4 min, also present in the plant, was detected in the strains expressing Ah AmaSy1 and Cc AmaSy1. The upward arrow (↑) indicates overexpression of the gene listed after the symbol. b Extracted-ion chromatogram (XIC) and the corresponding MS 2 spectrum from the LC-MS analysis revealed that this new compound with m / z = 713.2039 is bougainvillein-r I. The product ion at m / z = 389.09, characteristic for all betacyanins, corresponds to betanidin, the product ion at m / z = 343.09 corresponds to monodecarboxylated betanidin.

    Journal: Synthetic and Systems Biotechnology

    Article Title: Recombinant production of amaranthin and other betalain variants with yeast cell factories

    doi: 10.1016/j.synbio.2025.05.008

    Figure Lengend Snippet: Screening of glucuronosyltransferases in betanin-producing S. cerevisiae . a HPLC-UV chromatogram, comparing the total fraction of the parent strain ST12160 with the strains expressing Ah AmaSy1, Cq AmaSy1 or Cc AmaSy1 and the plant extract from B. glabra . A compound with an RT of 5.4 min, also present in the plant, was detected in the strains expressing Ah AmaSy1 and Cc AmaSy1. The upward arrow (↑) indicates overexpression of the gene listed after the symbol. b Extracted-ion chromatogram (XIC) and the corresponding MS 2 spectrum from the LC-MS analysis revealed that this new compound with m / z = 713.2039 is bougainvillein-r I. The product ion at m / z = 389.09, characteristic for all betacyanins, corresponds to betanidin, the product ion at m / z = 343.09 corresponds to monodecarboxylated betanidin.

    Article Snippet: To quantify the betalains produced by the yeast cultures, the samples were analysed via high-performance liquid chromatography (HPLC) using a Dionex Ultimate 3000 HPLC system (Thermo Fisher Scientific, US).

    Techniques: Expressing, Plant Extract, Over Expression, Liquid Chromatography with Mass Spectroscopy

    UDP-glucuronic acid synthesis in S. cerevisiae enables the production of amaranthin by glucuronosyltransferases . a Depending on the available sugar donor, betanin is glycosylated to bougainvillein-r I or amaranthin by the glucuronosyltransferases. At UGD1 converts UDP-glucose to UDP-glucuronic acid. b UDP-glucose and UDP-glucuronic acid concentrations in betalain producing S. cerevisiae without (ST12160) and with expression of a heterologous UDP-glucose dehydrogenase ( At UGD1). c HPLC analysis detects a new compound in all three strains expressing At UGD1 in combination with a glucuronosyltransferase that is also present in the plant extract from A. cruentus . LC-MS analysis confirms that this compound is amaranthin. d Betalain production in strains with or without At UGD1, quantified by HPLC. Concentrations of C15 stereoisomers were combined. Mean values of biological triplicates (±SD) are shown. “X” indicates the integration of At UGD1. e Betalain-producing S. cerevisiae strains on agar plate. At UGD1: UDP-glucose dehydrogenase ( A. thaliana ), Ah AmaSy1: Glucuronosyltransferase ( Amaranthus hypochondriacus ), Cq AmaSy1: Glucuronosyltransferase ( Chenopodium quinoa ), Cc AmaSy1: Glucuronosyltransferase ( Celosia argentea var. cristata ), Mj DOD: 4,5-extradiol dioxyge nase ( Mirabilis jalapa ), Bv TYH: tyrosine hydroxylase ( Beta vulgaris ), Bv GT2: UDP-glycosyltransferase ( Beta vulgaris ). The upward arrow (↑) indicates overexpression of the gene listed after the symbol.

    Journal: Synthetic and Systems Biotechnology

    Article Title: Recombinant production of amaranthin and other betalain variants with yeast cell factories

    doi: 10.1016/j.synbio.2025.05.008

    Figure Lengend Snippet: UDP-glucuronic acid synthesis in S. cerevisiae enables the production of amaranthin by glucuronosyltransferases . a Depending on the available sugar donor, betanin is glycosylated to bougainvillein-r I or amaranthin by the glucuronosyltransferases. At UGD1 converts UDP-glucose to UDP-glucuronic acid. b UDP-glucose and UDP-glucuronic acid concentrations in betalain producing S. cerevisiae without (ST12160) and with expression of a heterologous UDP-glucose dehydrogenase ( At UGD1). c HPLC analysis detects a new compound in all three strains expressing At UGD1 in combination with a glucuronosyltransferase that is also present in the plant extract from A. cruentus . LC-MS analysis confirms that this compound is amaranthin. d Betalain production in strains with or without At UGD1, quantified by HPLC. Concentrations of C15 stereoisomers were combined. Mean values of biological triplicates (±SD) are shown. “X” indicates the integration of At UGD1. e Betalain-producing S. cerevisiae strains on agar plate. At UGD1: UDP-glucose dehydrogenase ( A. thaliana ), Ah AmaSy1: Glucuronosyltransferase ( Amaranthus hypochondriacus ), Cq AmaSy1: Glucuronosyltransferase ( Chenopodium quinoa ), Cc AmaSy1: Glucuronosyltransferase ( Celosia argentea var. cristata ), Mj DOD: 4,5-extradiol dioxyge nase ( Mirabilis jalapa ), Bv TYH: tyrosine hydroxylase ( Beta vulgaris ), Bv GT2: UDP-glycosyltransferase ( Beta vulgaris ). The upward arrow (↑) indicates overexpression of the gene listed after the symbol.

    Article Snippet: To quantify the betalains produced by the yeast cultures, the samples were analysed via high-performance liquid chromatography (HPLC) using a Dionex Ultimate 3000 HPLC system (Thermo Fisher Scientific, US).

    Techniques: Expressing, Plant Extract, Liquid Chromatography with Mass Spectroscopy, Over Expression

    Expression of glucuronosyltransferases in betanin-producing Y. lipolytica . ST12603 and the GlcAT-expressing strains were cultivated in 24-well plates for 48 h. a Intra- and extracellular betalain concentrations, determined by HPLC. The corresponding 10x diluted samples are shown below the graph. Concentrations of the C15 stereoisomers were combined. Mean values of biological triplicates (±SD) are shown. b UDP-glucose and UDP-glucuronic acid concentrations in the yeast strains were measured by LC-MS. The upward arrow (↑) indicates overexpression of the gene listed after the symbol.

    Journal: Synthetic and Systems Biotechnology

    Article Title: Recombinant production of amaranthin and other betalain variants with yeast cell factories

    doi: 10.1016/j.synbio.2025.05.008

    Figure Lengend Snippet: Expression of glucuronosyltransferases in betanin-producing Y. lipolytica . ST12603 and the GlcAT-expressing strains were cultivated in 24-well plates for 48 h. a Intra- and extracellular betalain concentrations, determined by HPLC. The corresponding 10x diluted samples are shown below the graph. Concentrations of the C15 stereoisomers were combined. Mean values of biological triplicates (±SD) are shown. b UDP-glucose and UDP-glucuronic acid concentrations in the yeast strains were measured by LC-MS. The upward arrow (↑) indicates overexpression of the gene listed after the symbol.

    Article Snippet: To quantify the betalains produced by the yeast cultures, the samples were analysed via high-performance liquid chromatography (HPLC) using a Dionex Ultimate 3000 HPLC system (Thermo Fisher Scientific, US).

    Techniques: Expressing, Liquid Chromatography with Mass Spectroscopy, Over Expression

    Celoscristatin production in S. cerevisiae and Y. lipolytica . a To synthesise malonylated amaranthin from betanin, a glucuronosyltransferase ( Ah / Cq / Cc AmaSy1) and a malonyltransferase (e.g. Hp BAHD3) are required. b HPLC chromatogram (540 nm) of the extracellular fraction of amaranthin-producing S. cerevisiae strains, additionally expressing the acyltransferase Hp BAHD3. A new compound was detected in all three strains, absent in the reference strains. Y-axis: 10 – 120 mAU. The upward arrow (↑) indicates overexpression of the gene listed after the symbol. c Expression of Hp BAHD3 and Cc AmaSy1 in betanin-producing Y. lipolytica led to the formation of the same compound. From LC-MS analysis, this new compound was identified as celoscristatin (6′-O-malonyl-amaranthin) . d Phyllocactin- (ST14103), amaranthin- (ST14102) and celoscristatin- (ST14760) producing Y. lipolytica strains were cultivated in MM and the extracellular and intracellular production analysed by HPLC. Peak areas (mAU∗min) were compared. Mean values of biological triplicates (±SD) are shown. e UV–vis spectrum of 10x diluted extracellular and total samples. The corresponding 2x and 10x diluted samples of ST14760 are shown.

    Journal: Synthetic and Systems Biotechnology

    Article Title: Recombinant production of amaranthin and other betalain variants with yeast cell factories

    doi: 10.1016/j.synbio.2025.05.008

    Figure Lengend Snippet: Celoscristatin production in S. cerevisiae and Y. lipolytica . a To synthesise malonylated amaranthin from betanin, a glucuronosyltransferase ( Ah / Cq / Cc AmaSy1) and a malonyltransferase (e.g. Hp BAHD3) are required. b HPLC chromatogram (540 nm) of the extracellular fraction of amaranthin-producing S. cerevisiae strains, additionally expressing the acyltransferase Hp BAHD3. A new compound was detected in all three strains, absent in the reference strains. Y-axis: 10 – 120 mAU. The upward arrow (↑) indicates overexpression of the gene listed after the symbol. c Expression of Hp BAHD3 and Cc AmaSy1 in betanin-producing Y. lipolytica led to the formation of the same compound. From LC-MS analysis, this new compound was identified as celoscristatin (6′-O-malonyl-amaranthin) . d Phyllocactin- (ST14103), amaranthin- (ST14102) and celoscristatin- (ST14760) producing Y. lipolytica strains were cultivated in MM and the extracellular and intracellular production analysed by HPLC. Peak areas (mAU∗min) were compared. Mean values of biological triplicates (±SD) are shown. e UV–vis spectrum of 10x diluted extracellular and total samples. The corresponding 2x and 10x diluted samples of ST14760 are shown.

    Article Snippet: To quantify the betalains produced by the yeast cultures, the samples were analysed via high-performance liquid chromatography (HPLC) using a Dionex Ultimate 3000 HPLC system (Thermo Fisher Scientific, US).

    Techniques: Expressing, Over Expression, Liquid Chromatography with Mass Spectroscopy